Direct Competitive Enzyme-Linked Immunosorbent Assay for Sulfamethazine.

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition

ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL

An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...

enzyme-linked immunosorbent assay

An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...

Improved Direct Competitive Enzyme-Linked Immunosorbent Assay for Cyclopiazonic Acid in Corn, Peanuts, and Mixed Feed

An improved direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the analysis of cyclopiazonic acid (CPA) in corn, peanuts, and mixed feed. Two new approaches were used for the preparation of enzyme markers; one involved coupling CPA-bovine serum albumin (CPA-BSA) conjugate to horseradish peroxidase (HRP) using either the glutaraldehyde (GA) or the periodate (PI) me...

Enzyme-linked immunosorbent choriomammotropin assay.

We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assay...